Development of Inexpensive Multiplex Immunoassays: Assessment of Food Allergens in Plasma

1:45 AM 2:30 PM EXHIBITOR WORKSHOP ROOM 2 SCIENION US, Inc.

Presenter:

Attack by foreign substances, such as allergens, triggers a cascade of events, in which IgE is a first responder. An allergic reaction to certain foods, e.g., shellfish or peanuts, can elicit a response within minutes of ingestion. There are also more subtle responses, such as IgG, which remains in the bloodstream for an extended period, and is monitored to assess this delayed response. Th2 cytokines are involved in the humoral response, leading to the production ofIgE antibodies. Other cytokines, such as IL-25, are also involved in the induction of Th2 responses. In this study, a microplate-based microarray of allergenic proteins from food sources was constructed together with antibodies to measure inflammatory cytokines. Plasma from patients with known food allergy was assessed for specific IgG subclasses, specific IgE, and Th2 cytokine levels. Quantitative multiplex immunoassays were performed, and resulted in bright and colorful spots. The plates were analyzed using a novel colorimetric microplate imaging reader (sciREADER CL2, Scienion).

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IMMUNOLOGY 2017 May 12 16, 2017 | Washington, D.C.