Potential biomarker identified to screen quality of donor's stem cells before harvesting

Durham, NC - A new study released today in STEM CELLS addresses a significant problem that has been confronting human mesenchymal stem cells (hMSCs) therapy. While hundreds of clinical trials involving thousands of patients are under way to test hMSCs' ability to treat everything from heart disease to brain injury, there has been no way to determine prior to the donor undergoing a painful and expensive surgical harvesting of bone marrow whether or not it would be worth the effort. However, this new study, conducted by scientists at the Agency for Science, Technology and Research (A*STAR), Singapore, identifies a potential biomarker for prescreening donors for their MSCs' growth capacity and potency.

"With the global stem cell market predicted to reach over US$270 billion by 2025 (according to a report published by Transparency Market Research), there is a pressing need for effective biomarkers to be used in the screening of stem cells from prospective donors. This need is boosted by the rapid growth of regenerative medicine, with its pallet of cells, genes and engineered tissues," said Dr. Simon Cool, of A*STAR's Institute of Medical Biology and co-corresponding author of the study. That is what sparked this new investigation.

In an earlier study, this same laboratory had classified hMSCs from age and sex-matched human donors into high- and low-growth capacity groups and established criteria for identifying stem cells with enhanced potency. "These hMSCs showed increased proliferative potential that correlated with enhanced clonogenicity, a higher proportion of smaller-sized cells with longer telomeres, elevated expression of certain cell surface markers, and most importantly, improved ability to mediate ectopic bone formation," said the study's co-corresponding author, Lawrence Stanton, Ph.D., who at the time of the study was a member of A*STAR's Genome Institute of Singapore (and is now with Qatar Biomedical Research Institute).

The team's latest investigation sought to build upon that information by performing molecular analyses of these cells to better understand what accounted for their improved utility. Microarray analysis revealed that hMSCs with a genomic deletion of glutathione S-transferase theta (GSTT1) -- part of a superfamily of genes that bring together glutathione and toxins to safely remove them from the body -- show high-growth capacity. The GSTT1-null hMSCs also exhibit an enhanced ability to clone themselves and grow into full colonies, and they have longer telomeres. Both of these factors are important determinants of MSC potency.

"We believe our study highlights the utility of GSTT1 as a potential biomarker for MSC scalability and may prove useful in selecting potential donors for the creation of high quality hMSC cell banks to improve stem cell therapies," Dr. Cool said.

"The ability to pre-screen donors for a marker that corresponds to better growth of MSCs in vitro is truly important", said Dr. Jan Nolta, Editor-in-Chief of STEM CELLS. "Many teams have sought screening tools like this, which could prevent lot failure for clinical batches of MSCs that don't expand robustly. Until now, there has been no way to evaluate that prior to marrow harvest."

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The full article, "A Genomic Biomarker that Identifies Human Bone Marrow-Derived Mesenchymal Stem Cells with High Scalability," can be accessed at https://stemcellsjournals.onlinelibrary.wiley.com/doi/abs/10.1002/stem.3203.

About the Journal: STEM CELLS, a peer reviewed journal published monthly, provides a forum for prompt publication of original investigative papers and concise reviews. The journal covers all aspects of stem cells: embryonic stem cells/induced pluripotent stem cells; tissue-specific stem cells; cancer stem cells; the stem cell niche; stem cell epigenetics, genomics and proteomics; and translational and clinical research. STEM CELLS is co-published by AlphaMed Press and Wiley.

About AlphaMed Press: Established in 1983, AlphaMed Press with offices in Durham, NC, San Francisco, CA, and Belfast, Northern Ireland, publishes three internationally renowned peer-reviewed journals with globally recognized editorial boards dedicated to advancing knowledge and education in their focused disciplines. STEM CELLS is the world's first journal devoted to this fast paced field of research. THE ONCOLOGIST is devoted to community and hospital-based oncologists and physicians entrusted with cancer patient care. STEM CELLS TRANSLATIONAL MEDICINE is dedicated to significantly advancing the clinical utilization of stem cell molecular and cellular biology. By bridging stem cell research and clinical trials, SCTM will help move applications of these critical investigations closer to accepted best practices.

About Wiley: Wiley, a global company, helps people and organizations develop the skills and knowledge they need to succeed. Our online scientific, technical, medical and scholarly journals, combined with our digital learning, assessment and certification solutions, help universities, learned societies, businesses, governments and individuals increase the academic and professional impact of their work. For more than 200 years, we have delivered consistent performance to our stakeholders. The company's website can be accessed at http://www.wiley.com.

This story has been published on: 2020-06-08. To contact the author, please use the contact details within the article.

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