IMMUNOLOGY AND INFLAMMATION Correction for TAp73 represses NF-Bmediated recruitment of tumor-associated macrophages in breast cancer, by Johanna Wolfsberger, Habib A. M. Sakil, Leilei Zhou, Niek van Bree, Elena Baldisseri, Sabrina de Souza Ferreira, Veronica Zubillaga, Marina Stantic, Nicolas Fritz, Johan Hartman, Charlotte Rolny, and Margareta T. Wilhelm, which published March 1, 2021; 10.1073/pnas.2017089118 (Proc. Natl. Acad. Sci. U.S.A. 118, e2017089118).
The authors note that Supporting Table S2 in the SI Appendix appeared incorrectly. Regarding this error, the authors note, Sanger sequencing primers for the mouse and human Ccl2 gene have mistakenly been entered in the qPCR primer table (SI Appendix, Table S2) instead of the qPCR primer sequences for Ccl2 that were used in this study.
The authors also note that an error appeared in Fig. 3 and Fig. S3 in the SI Appendix. In both figures, the histograms are labeled DMSO but should instead be labeled siCtrl. The corrected figure and its legend appear below. The online version has been corrected, including the SI Appendix with the corrected Table S2 and Fig. S3.
TAp73 represses NF-Bmediated regulation of Ccl2 expression. (A) qRT-PCR analysis of Ccl2 mRNA levels in (A) TAp73 WT and KO MEFE1A/Ras (n = 3) and (B) PyMT/TAp73 WT or KO cells (n = 4) after 16-h treatment with NF-B inhibitor SC-514 (100 g/mL). ELISA analysis of Ccl2 protein secretion in conditioned media from (C) TAp73 WT and KO MEFE1A/Ras (n = 3) and (D) PyMT/TAp73 WT or KO cells (n = 4) after 16-h treatment with SC-514 (100 g/mL). (E and F) qRT-PCR analysis of Ccl2 mRNA levels in (E) TAp73 WT and KO MEFE1A/Ras (n = 3) and (F) PyMT/TAp73 WT or KO cells (n = 3) after 48-h treatment with sip65/RELA. (G and H) ELISA analysis of Ccl2 protein secretion in conditioned media from (G) TAp73 WT and KO MEFE1A/Ras (n = 3) and (H) PyMT/TAp73 WT or KO cells (n = 3) after 48-h treatment with sip65/RELA. (I) Schematics showing the murine Ccl2 promoter including NF-B response elements, of which the distal BSs 3 and 4 were deleted either individually or simultaneously. (J) WT or mutated Ccl2 promoter luciferase reporter was cotransfected with TAp73 in HEK293 cells. Luciferase activity was measured 24 h after transfection and normalized to mock control (n = 6). All data are shown as mean SD *P < 0.05, **P < 0.01, ****P < 0.0001.
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Correction for Wolfsberger et al., TAp73 represses NF-Bmediated recruitment of tumor-associated macrophages in breast cancer - pnas.org
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