Background: Sepsis is a common clinical syndrome in critical patients in the medical intensive care unit. Many scoringsystems and biomarkers are introduced to detect unfavorable outcomes in sepsis patients. This study aims to identify pentraxin 3 (PTX3) as a predictor of sepsis in patients who are critically ill and admitted to intensive care units.

Materials and methods: This prospective observational survey purposively included 100 patients with sepsis identified by the Surviving Sepsis Campaign guidelines in the medical intensive care unit at one of the apex care centers in North India. Socio-demographic and clinical profiles were collected using a structured and validated checklist. Simple and multi-linear regression analyses were used to determine PTX3 as a predictor of sepsis.

Results: A total of 100 patients were prospectively observed. Among them, 61% were males, and 39% were females, with a mean age of 50.78 (13.53) years. From nine potential predictors, lactate (95% CI: 1.048-1.890, B: 1.469, p < 0.001), procalcitonin (95% CI: 0.136-0.270, B: 0.203, p < 0.001), andSOFA (Sequential Organ Failure Assessment) scores (95% CI: 0.112-0.450, B: 0.281, p = 0.001) significantly predictthe changes in PTX3 level (R-square: 0.842, adjusted R-square:0.826) in patients.

Conclusions: PTX3was found to correlate with the severity of sepsis as SOFA scoreand other markers like lactate, procalcitonin, and APACHE-II (Acute Physiology and Chronic Health Evaluation II) score.

Sepsis is one of the most common clinical syndromes caused by systemic infection and often leads to a lethal outcome in critically ill patients. The Global Sepsis Alliance recommendation defined sepsis as a condition of life-threatening organ dysfunction caused by the dysregulated host response to infection. It has been a significant cause of intensive care unit admission worldwide.Sepsis results from the complicated interactions between the host immune system and infecting viruses and bacteria [1]. The mortality associated with sepsis is significant around the globe and is more than breast and lung cancer altogether. Furthermore, a lack of specific treatment for sepsis results in higher incidence and many complications, including septic shock, multiple organ dysfunction syndrome, and death. Multiorgan dysfunction and septic shock are the most common cause of mortality in patients with sepsis [2].

Further, diagnosing sepsis remains a significant challenge for health professionals considering concurrent organ support, organ dysfunction, treatment before admission, and lack of a "gold standard" diagnostic test. Over the advancement in medical sciences, many scoring systems were introduced as a surrogate for organ dysfunction risk prediction for patients with proven or suspected infection, including SOFA (Sequential Organ Failure Assessment) and APACHE-II (Acute Physiology and Chronic Health Evaluation II), to determine the degree of organ dysfunction and severity of disease in chronically ill patients [3-5]. Likewise, many biomarkers have been proposed for risk prediction in severely ill patients. Procalcitonin (PCT) [6], serum lactate [7], and cytokines are studied in patients with sepsis and shock. The inflammatory process in the body leads cytokines to produce higher PCT from the liver and mononuclear cells and subsequently increases the level in the body [8].

Higher levels of plasma lactate have been considered an essential indicator of hemodynamic stability. Breakthrough work has been conducted on the role of serum lactate on the survival of critically ill patients, reflecting a higher level of lactate decreases survival. Earlier research on hemodynamically stable patients reported a higher lactate level in non-survivors of emergencies. A higher lactate level is common among chronically ill patients and is recommended as a reliable marker of illness severity and death [9,10].

Pentraxin 3(PTX3) isan acute-phase protein that represents the subfamily of long pentraxin [11]. Ithas been found to have a strong association with the severity of infection and inflammation. The inflammatory process initiates the secretion of PTX3 in monocytes, endothelial cells, and dendritic or neutrophils [12].

Numerous studies done to date have noticed that PTX3 has an excellent diagnostic value in sepsis. PTX3 as a biomarker of sepsis, and its diagnostic utility in northern Indian tertiary care setup, would help us evaluate the future scope in patients with sepsis. A crunch of studies, none to the best of our knowledge, in the Indian subcontinent further emphasizes the need to assess the diagnostic utility of PTX3 inpatients with sepsis. Therefore, we decided to conduct a survey to correlate serum PTX3 levels with the severity of sepsis.

This is a cross-sectional studyconducted over 18 months at All India Institute of Medical Sciences (AIIMS) Rishikesh, a tertiary healthcare center in Uttarakhand, India.

The minimum sample size required for the study was estimated by using Fishers transformation formula, which came out to be 95. However, the authors decided to enroll 100 patients consecutively at the in-patient department of general medicine. The patients fulfilling the Surviving Sepsis Campaign guidelines and willing to write consent were included till the sample size was achieved. Patients who were on steroid therapy, diagnosed with immunodeficiency disorders, pregnant, and diagnosed with tuberculosis and acute coronary artery disease were excluded from the study (Figure 1).

Socio-demographic details and baseline investigations were recorded using a structured and validated checklist. SOFA score and APACHE-II scores were calculated. PCT was measured by chemiluminescence on the Advia Centaur instrument (Siemens Healthineers, Erlangen, Germany), and lactate was measured by arterial blood sample using a blood gas analyzer (ABL800, Radiometer, Copenhagen, Denmark). Blood samples were collected into plain and ethylenediaminetetraacetic acid (EDTA) vials. All the blood samples were subjected to centrifugation at 2500g for 10 minutes at 4C within 30 minutes of blood sampling.Plasma and serum got separated and aliquoted. The aliquoted samples were collected and stored until analysis at -80C. Pentraxin was analyzed by Sandwich ELISA (ImmunoTag, St. Louis, MO) following the manufacturers instruction after obtaining a satisfactory standard curve.

The Institutional Ethics Committee of All India Institute of Medical Sciences Rishikesh (AIIM/IEC) approved the project (AIIMS/IEC/20/575). Written informed consent was obtained from each participant before enrolling in the study. Participants were ensured to protect privacy and confidentiality at each stage of research.

Data were transferred to a Microsoft Excel sheet (Microsoft Corporation, Redmond, WA) and analyzed by using Statistical Package for the Social Sciences (SPSS) version 26.0 (IBM Corp., Armonk, NY). Frequency, percentage, means, and standard deviation(SD) were used to describe the patients characteristics. Categorical variables were represented as proportions. Continuous or discrete variables were reported using means and SD. Spearman correlation coefficient was used to find the correlation between PTX3 and other continuous variables considering the non-normal distribution of the variables. Mann-Whitney U test was used to compare the distribution of mean PTX3 levels with genders. Simple andmultivariate regression analyses were done to find out whether PTX3 is a predictor of sepsis level. All test statistics were measured at p < 0.05 level (two-tailed).

A total of 100 participants were included in the study; among them, 61.0% were males and 39% were females. The mean age of the patients was 50.78 (SD = 13.53) years. Of the participants, 63% had one or more comorbidities, 21% had only diabetes mellitus, 21% had only hypertension, and 21% had both hypertension and diabetes mellitus (Appendix A). The mean SOFA score was 8.94 (SD = 2.86), the mean APACHE-II score was 14.48 (SD = 5.05), and the mean PXT3 value was 5.24 (SD = 3.38) ng/ml. Further, the PXT3 level did not show any significant difference with comorbidity status (p = 0.134) (Appendix B). Similarly, results show no significant difference in PXT3 levels among males and females (U = 982, p = 0.142). Serum lactate (r = 0.661, p < 0.001) and PCT (r = 0.663, p < 0.001) reported significant association with SOFA scores (Table 1).

Considering the non-normal distribution of PXT3 among patients, Spearmans rho test was applied to find an association with the SOFA scores. The findings show a significant positive correlation of PXT3 with the fraction of inspired oxygen (FiO2), partial pressure of carbon dioxide (PCO2), lactate, PCT, and blood urea. Further, the PXT3 level reported a significant positive relationship with the SOFA score (r = 0.722) and APACHE-II scores (r = 0.393) (Table 2).

Simple linear regression analysis depicted that out of nine potential variables, seven variables, includingFiO2 (p < 001), partial pressure of oxygen (PaO2) (p = 0.004), PCO2 (p = 0.034), PCT(p < 0.001, R-square, 0.657), lactate (R-square, 0.635), SOFA (p < 0.001, R-square 0.610), and APACHE-II(p < 0.001), highly predicted the change in PXT3 (Table 3 and Figure 2).

Multivariate linear regression analysis was used to combine the cumulative effect of significant variables of simple linear regression. Findings reported that lactate (95% CI: 1.048-1.890, B: 1.469, p <0.001), PCT (95% CI: 0.136-0.270, B: 0.203, p < 0.001), and SOFA score (95% CI: 0.112-0.450, B: 0.281, p = 0.001) significantly predictthe change in PXT3 level (R-square: 0.842; adjusted R-square: 0.826)(Table 4).

Sepsis is a major cause of mortalityworldwide, especially in developing countries, including India, considering the lack of protocolized care and crunch of resources [13]. It remains the major cause of mortality among critically ill patients. It has been demonstrated in previous work that early identification and protocol-based treatment of severe sepsis can improve the survival of patients.In recent years, there are many novel biomarkers, including PTX3, C-reactive protein (CRP), PCT, and plasma PTX3, that are identified to early anticipate sepsis and plan treatment [14]. However, none of the single biomarkers is ideal and helpful in identifying critically ill patients with their respective drawbacks.This study analyzed the PTX3 level in critically ill patients with sepsis and its correlation with lactate and PCT and critical illness index, includingSOFA score and APACHE-II score.

The mean age of participants (n = 100) was 50.78 (13.524) years with a predominantly male population. The proportion of participants with either hypertension or diabetes only was equal. The mean arterial blood pressure of the patients was 67.01 (10.518) mmHg. Most patients had lungs as the source of infection, followed by abdominal infection and urinary tract infection. These findings were in line with most of the trials,including those byChatterjee et al., which were done in India where 53% of participants had lungs as the primary source [15]. Other sites included skin, bacteremia, etc., which were less commonly seen in our setting. Of 100 participants, only 68 had positive cultural results. Most of them had gram-negative bacteria in their culture, which included Acinetobacter, Pseudomonas, and Klebsiella species.

The relevant laboratory parameters were measured, and the SOFA score and APACHE-II score were calculated. We analyzed the relationship between PTX3 and various laboratory parameters. We observed that blood pH and PaO2have a negative correlation with PTX3, whereas FiO2, PCO2, lactate, PCT, and blood urea had a positive correlation with PTX3.

The primary objective of the study was toanalyze PTX3 levelsand see the correlation with SOFA scores.The study findings reported that lactateand PCT show a significant correlation with PTX3 in the studied cohort. These findings are in accord with several previously published studies that reported a significant relationship of PTX3 with lactate and PCT[16,17].

The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3) recommended measuring lactate levels for septic shock [18]. It has been suggested that serum lactate levels canbe used to screen for sepsis among adults with clinical suspicion of sepsis. Several studies were conducted toassess the use of lactate in this context [19,20]. We have also included lactate in our study, inwhich lactate was found to correlate with SOFA score, which is similar to the study done by Liu et al. in 2019 [16]. In ourstudy, we have also found that PTX3 is positively correlated with lactate (p = 0.000). This is in line with a previous prospective study done byHu et al. [14]. However, serum lactate alone is neither sensitive nor specific to rule in or rule out the diagnosis of sepsis on its own. Thetestingof lactate may not be available in resource-limited settings [21]. Hence, it was given as a weak recommendation to use lactate levels in serum as an adjunctive test to detect the probability of sepsis in patients with suspected but not confirmed sepsis.

PCT fulfills the needfor high diagnostic accuracy in detecting sepsis, which is needed to be used as a biomarker, especially in comparison to conventional. PCT alonecannot identify specific pathogens of sepsis, but the level of PCT might be useful to estimate the probability of severe bacterial infection [22].We measured the level of PCT of all the participants, the mean of which was 9.7 (SD: 6.4), and it was found to correlate with the SOFA score.

A prospective study done by Sudhir et al. depicted thatthere was a significant association between PCTand SOFA score [23]. But in a retrospective cohort study done by Yunus et al., there was a weak correlation between PCT and SOFA score [24].In our study, PTX3 has a significant positive correlation with PCT with a p-valueof 0.000 (r = 0.856), which is similar to the previous study[25].

APACHE-II scoreis one of the scoring systems used to determinethe severity of disease and predict the mortality of sepsis patients. Hillet al., in a pilot study, found thatPTX3 levels were increased in patients with sepsis and are related to APACHE-II scores when plotted according to the APACHE-II score quartile [26]. Our study found that PTX3levels are correlating significantly with the APACHE-II score (p = 0.00).

We didmultistep-wise forward linear regression analysis and interestingly we found that the best individual marker to predict PTX3 is PCT with an R-squareof 0.657 andan adjusted R-square of 0.654, with p = 0.00. The predictability increased with PCT and lactate together with an R-square of 0.808 and an adjustedR-square of0.804 significantly (p = 0.00). The predictability of PTX3 is further increased with PCT, lactate, and SOFA scores together significantly with an R-square of 0.830 and an adjusted R-square of 0.825.

The efficacy of PTX3 as a biomarker tool in sepsis has been demonstrated in the work of many studies. In the Albumin Italian Outcome Sepsis (ALBIOS) trial, which is a multi-centric trial done on 1818 patients,it was found thatPTX3levels areelevated in severe sepsisandcorrelate significantlywith prevalent and incident organ failures [27].Similarly, in a prospective study done by Uusitalo-Seppl et al., it was found that measuring PTX3 level at admission highly predicts severe sepsis and case fatality [28]. Lee et al., in a meta-analysis, found that PTX3 significantly predicts the severity of the diseaseand mortality in sepsis [29].

Hamed et al. conducted the Mannheim Sepsis Study, which is a prospective, monocentric study done on 217 intensive care unit patients, included according to the latest Sepsis-3 definitions. This study demonstrated that PTX3 exhibits potential diagnostic value in comparison to CRPand interleukin-6, and PTX3 is correlating with SOFA score [12].

Albeit, PCT is a promising marker of infectionand due to its early rise and short half-life, the studies are heterogeneous and lackconsensus. Lactate is a marker detecting endogenous catecholamine release. Patients who are maintaining their blood pressure due to a vigorous catecholamine response may have deceptively reassuring vital signs and maskthe catecholamine-dependent shock. Elevated lactate identifies these patients having occult shock who are more at risk of adverse outcomes so each of the three biomarkers plays a crucial role in helping the management of sepsis.

Akin to most of the studies, we found that PTX3 correlates with disease severity scores SOFA and APACHE-II. PTX3 is also correlating significantly with PCT and lactate, biomarkers found to be effective in sepsis for ages. We also found that PCT, lactate, andSOFA scores togetherpredicted PTX3 significantly, and the predictability is better than individual components. Among PCT, lactate, and SOFA score, the best predictor of PTX3 is PCT. PTX3, in combination with established other markers, might improve the correlation with sepsis severity and needs to be studied.

Hence, the novel marker PTX3 with its advantages needs to be considered and to be studied in future studies, as sepsis is one of the most common causes of mortality in humankind, which should be emphasized.

Thisis a single-centerstudy, and we have not included the mortality data; hence, an association between PTX3 and mortality cannot be established. In this survey, we measured PTX3 once, which may not be sufficient to conclude, and the authors recommend a longitudinal large-scale study to understand the exact role of PTX3 in sepsis development and other health consequences. A multi-centric randomized controlled trial might recheck the results of the present work. A study with a higher sample size is recommended to improve generalizability over other similar populations.

Currently, the accuracy of various biomarkers in sepsis and septic shock and their correlation with severity has thrown open wide conflicting results with many studies showing different results. PTX3was analyzed and studied according to the latest Sepsis-3 guidelines in our study and was found to correlate with the severity of sepsis as SOFA scoreand other markers like lactate and PCT along with APACHE-II score.Our study did not permit us to rush to the conclusion of whether PTX3 is a better marker compared to other biomarkers in sepsis in use now. It raised the question of whether PTX3 can be used as a tool in sepsis for early detection. To find a definitive answer, larger randomized control trials are needed.

The study correlated PTX3 levels with PCT and lactate, which are age-old markers established in sepsis, unlike other new markers. Second multi-stepwise forward linear regression analysis was done to see better predictors of PTX3, adjunctively demonstrating its correlation with sepsis. Henceforth, novel biomarkers such as PTX3 in combination with lactate, PCT, and SOFA score might be helpful to improve the risk stratification of patientswith sepsis.

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Pentraxin 3 (PTX3) as a Predictor of Severity of Sepsis in Patients Admitted to an Intensive Care Unit: A Cross-Sectional Study From North India -...